DNA

Part:BBa_K4687023:Design

Designed by: Zhimin Zhan   Group: iGEM23_HBUT-China   (2023-10-02)


Donor:crtEb/crtR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3148
    Illegal PstI site found at 2652
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3148
    Illegal NheI site found at 2265
    Illegal PstI site found at 2652
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3148
    Illegal XhoI site found at 1966
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3148
    Illegal PstI site found at 2652
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3148
    Illegal PstI site found at 2652
    Illegal NgoMIV site found at 3563
    Illegal AgeI site found at 1892
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

After cleaving the DNA gap by CRISPR-MAD7 nuclease, the donor DNA undergoes homologous recombination repair to achieve the knockout of the targeted gene.


Source

It is derived from a continuous random sequence of 1000bp in length with homology effects at the front and end of the CRISPR-MAD7 nuclease binding to the PAM region.

References